microRNA（miRNA）是一类由内源基因编码、长度约为22个核苷酸的非编码单链RNA分子，在细胞分化、生物发育及疾病发展过程中发挥关键的调控作用。
赛索飞生物拥有经验丰富且专业的寡核苷酸合成技术团队，成功开发了用于功能获得研究的miRNA模拟物和用于功能丧失研究的miRNA抑制剂，并针对细胞实验和动物实验提供定制化方案。
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应用案例
01. miRNA-146b-5p Inhibitor和Mimic在TNBC细胞中的功能验证
图1. 细胞活力检测
图2. 细胞凋亡检测
Ref: Dai X et al., Int J Biol Macromol (2025): 144142.
miRNA-146b-5p Mimic能显著抑制三阴性乳腺癌 (TNBC)细胞活力并促进细胞凋亡，而Inhibitor则表现出相反的作用。
02. miR-9-5p在小鼠脑源性外泌体中的功能探索
图3. miR-9-5p表达水平的RT-qPCR分析
Ref: Zhang Y et al., CNS Neurosci Ther 30.12 (2024): e70189.
antagomir-9-5p可显著抑制提取的外泌体（图3左）和注射外泌体后的小鼠肺组织（图3右）中miR-9-5p的活性。
客户文章
1. IF：8.5
Cold atmospheric plasma targets triple negative breast cancer cells via SCAF11-mediated competitive protein degradation and synergizes with miRNA-146b-5p
客户单位：西安交通大学第一附属医院、江南大学
发表期刊：International Journal of Biological Macromolecules
文章链接:
www.sciencedirect.com/journal/international-journal-of-biological-macromolecules
引用内容
miRNA mimic & inhibitor: “The miRNA-146b-5p mimics (3’-UGAGAACUGAAUUCCAUAGGC
UG-5′) and inhibitor (5’-mCmAmGmCmUmAmUmGmGmAmAmUmUmCmAmGmUmUmCmA-3′) were designed and purchased from GenCefe Biotech Co., Ltd.”
2. IF：5.5
Black Phosphorus Nanosheets for Enhanced Osteogenic Differentiation of Dental Follicle Stem Cells
客户单位：青岛大学附属医院
发表期刊：ACS Applied Nano Materials
文章链接:
pubs.acs.org/doi/10.1021/acsanm.6c00876
引用内容
miRNA Inhibitor & mimic、转染试剂: “When cells reached ∼70% confluence, transfection was performed using Gencefe TransExp (Gencefe, China) according to the manufacturer’s instructions.” “The miR-8085 mimic, mimic negative control (mimic NC), miR-8085 inhibitor, and inhibitor negative control (inhibitor NC) were synthesized by Gencefe (China).”
3. IF：5.3
circ-1584 selectively promotes the antitumor activity of the oncolytic virus M1 on pancreatic cancer
客户单位：中山大学、广东省人民医院
发表期刊：Molecular Therapy Oncology
文章链接:
www.cell.com/molecular-therapy-family/oncology/fulltext/S2950-3299(24)00161-9#au1
引用内容
基因合成、siRNA、miRNA: The sequence of human circ-1584 was generated through chemical gene synthesis and cloned into the lentiviral vectors (LV, pCDH-CMV-MCS-EF1-PURO) and pcDNA3.1 plasmid (GENCEFE, Wuxi, China) to construct the overexpression vectors. siRNAs specific to human circ-1584 were synthesized at GENCEFE and used to transiently silence the expression of circ-1584. Mimics of miR-578 and NC mimics were synthesized at GENCEFE.
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